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mixmate microplate shaker  (Eppendorf AG)


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    Structured Review

    Eppendorf AG mixmate microplate shaker
    Mixmate Microplate Shaker, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 203557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mixmate+microplate+shaker/Eppendorf/pmc12796099-279-41-49
    Average 99 stars, based on 203557 article reviews
    mixmate microplate shaker - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: CRISPR-addressable yeast strains with applications in human G protein–coupled receptor profiling and synthetic biology
    Article Snippet: .. Plates with normalized cultures were covered with porous film (Diversified Biotech; catalog no. BERM-2000), shaken (1200 rpm for 30 s) on a MixMate microplate shaker (Eppendorf, Hamburg, Germany), and incubated at 30 °C for ≈18 h. Data acquisition All data were collected from four biological replicates ( i.e. colonies), the fluorescence of which was measured over a linear dilution series. ..

    Article Title: The evolution and mechanism of GPCR proton sensing
    Article Snippet: A Biomek NX P liquid-handling robot was used to prepare 50 μl of cell cultures normalized to an A 600 nm of 0.05 in black 384-well clear-bottom plates (Greiner Bio-One; catalog no. 781096). .. The plates were covered with a porous film (Diversified Biotech; catalog no. BERM-2000), shaken (1200 rpm for 30 s) on a MixMate microplate shaker (Eppendorf, Hamburg, Germany), and incubated at 30 °C for ≈22 h. Fluorescence (instrument gain of 1300) and A 600 nm measurements were taken at 2 to 3 h increments. ..

    Article Title: The evolution and mechanism of GPCR proton sensing
    Article Snippet: A Biomek NXP liquid-handling robot was used to prepare 50 μL of cell cultures normalized to an A600 nm of 0.05 in black 384-well clear-bottom plates (Greiner Bio-One; catalog no. 781096). .. Plates were covered with porous film (Diversified Biotech; catalog no. BERM-2000), shaken (1200 rpm for 30 s) on a MixMate microplate shaker (Eppendorf; Hamburg, Germany), and incubated at 30°C for » 22 hours. ..

    Fluorescence:

    Article Title: CRISPR-addressable yeast strains with applications in human G protein–coupled receptor profiling and synthetic biology
    Article Snippet: .. Plates with normalized cultures were covered with porous film (Diversified Biotech; catalog no. BERM-2000), shaken (1200 rpm for 30 s) on a MixMate microplate shaker (Eppendorf, Hamburg, Germany), and incubated at 30 °C for ≈18 h. Data acquisition All data were collected from four biological replicates ( i.e. colonies), the fluorescence of which was measured over a linear dilution series. ..

    Article Title: The evolution and mechanism of GPCR proton sensing
    Article Snippet: A Biomek NX P liquid-handling robot was used to prepare 50 μl of cell cultures normalized to an A 600 nm of 0.05 in black 384-well clear-bottom plates (Greiner Bio-One; catalog no. 781096). .. The plates were covered with a porous film (Diversified Biotech; catalog no. BERM-2000), shaken (1200 rpm for 30 s) on a MixMate microplate shaker (Eppendorf, Hamburg, Germany), and incubated at 30 °C for ≈22 h. Fluorescence (instrument gain of 1300) and A 600 nm measurements were taken at 2 to 3 h increments. ..

    Transformation Assay:

    Article Title: PIONEER: A Periplasmic Display Platform for Synthetic Biology-Based Screening of Genetically Encoded Protein Regulators
    Article Snippet: A CLARIOstar multimode microplate reader (BMG LabTech) was used for collecting all microplate-based absorbance at 600 nm ( A 600 ), temperature, fluorescence (RFU), and luminescence (RLU) data with the following instrument settings: A 600 (excitation 600 nm; 22 flashes/well), mTq2-based fluorescence (excitation 430/10 nm, dichroic LP 458 nm, emission 482/16 nm; 10 flashes/well; bottom read), NanoLuc luminescence (emission 482/16 nm; top read). .. Yeast cells transformed with pYEplac181 αPre-mTq2- X , where X is the display candidate, were picked from SCD-LEU agar medium into 500 uL SCD-LEU medium in sterile 96-well deep-well blocks (Greiner Bio-One), covered with porous film (Diversified Biotech), shaken on a MixMate microplate shaker (1200 rpm for 30 sec) (Eppendorf), and placed at 30°C. ..

    Article Title: PIONEER: A periplasmic display platform for synthetic biology-based screening of genetically encoded protein regulators
    Article Snippet: A CLARIOstar multimode microplate reader (BMG LabTech) was used for collecting all microplate-based absorbance at 600 nm ( A 600 ), temperature, relative fluorescence units (RFU), and relative luminescence units (RLU) data with the following instrument settings: A 600 (excitation 600 nm; 22 flashes/well), mTq2-based fluorescence (excitation 430/10 nm, dichroic LP 458 nm, emission 482/16 nm; 10 flashes/well; bottom read), NanoLuc luminescence (emission 482/16 nm; top read). .. Yeast cells transformed with pYEplac181 αPre-mTq2- X , where X is the display candidate, were picked from SCD-LEU agar medium into 500 uL SCD-LEU medium in sterile 96-well deep-well blocks (Greiner Bio-One), covered with porous film (Diversified Biotech), shaken on a MixMate microplate shaker (1200 rpm for 30 s) (Eppendorf), and placed at 30 °C. .. After 18 h, cultures were used to prepare new 500-uL cultures normalized to A 600 0.2 and allowed to grow at 30 °C for 3 h. Finally, 50-uL cultures normalized to A 600 0.1 were prepared in a black 384-well clear-bottom plate (Greiner Bio-One) and placed in a microplate reader programmed for 30 °C incubation.

    Article Title: PIONEER: A Periplasmic Display Platform for Synthetic Biology-Based Screening of Genetically Encoded Protein Regulators.
    Article Snippet: A CLARIOstar multimode microplate reader (BMG LabTech) was used for collecting all microplate-based absorbance at 600 nm (A600), temperature, fluorescence (RFU), and luminescence (RLU) data with the following instrument settings: A600 (excitation 600 nm; 22 flashes/well), mTq2-based fluorescence (excitation 430/10 nm, dichroic LP 458 nm, emission 482/16 nm; 10 flashes/well; bottom read), NanoLuc luminescence (emission 482/16 nm; top read). .. Yeast cells transformed with pYEplac181 Pre-mTq2-X, where X is the display candidate, were picked from SCD-LEU agar medium into 500 uL SCD-LEU medium in sterile 96-well deep-well blocks (Greiner Bio-One), covered with porous film (Diversified Biotech), shaken on a MixMate microplate shaker (1200 rpm for 30 sec) (Eppendorf), and placed at 30C. ..

    Sterility:

    Article Title: PIONEER: A Periplasmic Display Platform for Synthetic Biology-Based Screening of Genetically Encoded Protein Regulators
    Article Snippet: A CLARIOstar multimode microplate reader (BMG LabTech) was used for collecting all microplate-based absorbance at 600 nm ( A 600 ), temperature, fluorescence (RFU), and luminescence (RLU) data with the following instrument settings: A 600 (excitation 600 nm; 22 flashes/well), mTq2-based fluorescence (excitation 430/10 nm, dichroic LP 458 nm, emission 482/16 nm; 10 flashes/well; bottom read), NanoLuc luminescence (emission 482/16 nm; top read). .. Yeast cells transformed with pYEplac181 αPre-mTq2- X , where X is the display candidate, were picked from SCD-LEU agar medium into 500 uL SCD-LEU medium in sterile 96-well deep-well blocks (Greiner Bio-One), covered with porous film (Diversified Biotech), shaken on a MixMate microplate shaker (1200 rpm for 30 sec) (Eppendorf), and placed at 30°C. ..

    Article Title: PIONEER: A periplasmic display platform for synthetic biology-based screening of genetically encoded protein regulators
    Article Snippet: A CLARIOstar multimode microplate reader (BMG LabTech) was used for collecting all microplate-based absorbance at 600 nm ( A 600 ), temperature, relative fluorescence units (RFU), and relative luminescence units (RLU) data with the following instrument settings: A 600 (excitation 600 nm; 22 flashes/well), mTq2-based fluorescence (excitation 430/10 nm, dichroic LP 458 nm, emission 482/16 nm; 10 flashes/well; bottom read), NanoLuc luminescence (emission 482/16 nm; top read). .. Yeast cells transformed with pYEplac181 αPre-mTq2- X , where X is the display candidate, were picked from SCD-LEU agar medium into 500 uL SCD-LEU medium in sterile 96-well deep-well blocks (Greiner Bio-One), covered with porous film (Diversified Biotech), shaken on a MixMate microplate shaker (1200 rpm for 30 s) (Eppendorf), and placed at 30 °C. .. After 18 h, cultures were used to prepare new 500-uL cultures normalized to A 600 0.2 and allowed to grow at 30 °C for 3 h. Finally, 50-uL cultures normalized to A 600 0.1 were prepared in a black 384-well clear-bottom plate (Greiner Bio-One) and placed in a microplate reader programmed for 30 °C incubation.

    Article Title: PIONEER: A Periplasmic Display Platform for Synthetic Biology-Based Screening of Genetically Encoded Protein Regulators.
    Article Snippet: A CLARIOstar multimode microplate reader (BMG LabTech) was used for collecting all microplate-based absorbance at 600 nm (A600), temperature, fluorescence (RFU), and luminescence (RLU) data with the following instrument settings: A600 (excitation 600 nm; 22 flashes/well), mTq2-based fluorescence (excitation 430/10 nm, dichroic LP 458 nm, emission 482/16 nm; 10 flashes/well; bottom read), NanoLuc luminescence (emission 482/16 nm; top read). .. Yeast cells transformed with pYEplac181 Pre-mTq2-X, where X is the display candidate, were picked from SCD-LEU agar medium into 500 uL SCD-LEU medium in sterile 96-well deep-well blocks (Greiner Bio-One), covered with porous film (Diversified Biotech), shaken on a MixMate microplate shaker (1200 rpm for 30 sec) (Eppendorf), and placed at 30C. ..



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